Primer3 0.4.0 __full__ -

The default salt correction formula and mismatched pair penalty parameters have been (SantaLucia & Hicks, 2004; von Ahsen et al., 2001). This leads to slightly more accurate Tm predictions for:

From castor bean U6 promoters to human mitochondrial DNA , the software’s flexibility makes it a universal tool across the biological sciences. Key Applications in Modern Science primer3 0.4.0

Version 0.4.0 uses simpler, predictable calculations. For standard, non-multiplex PCR reactions, its selections are highly reliable. The default salt correction formula and mismatched pair